The Monkey Anti Tetanus Toxoid IgG1 ELISA Kit is based on a solid phase enzyme-linked immunosorbent assay (ELISA). The assay uses tetanus toxoid for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated mouse monoclonal anti-monkey IgG1 antibody for detection.. Strip well format. Reagents for up to 96 tests.

The monkey anti-tetanus toxoid IgG test kit is based on a solid phase enzyme-linked immunosorbent assay (ELISA). The assay uses tetanus toxoid for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated mouse monoclonal anti-monkey IgG1 antibody for detection. Serum or plasma samples are diluted and incubated in the microtiter wells for 60 minutes. The microtiter wells are subsequently washed, and HRP conjugate is added and incubated for 45 minutes. Anti-tetanus toxoid IgG1 molecules are thus sandwiched between immobilized tetanus toxoid and the detection antibody conjugate. The wells are then washed to remove unbound HRP-labeled antibodies, and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow, and optical density is measured spectrophotometrically at 450 n

More Details:

• Detection Target: Anti Tetanus Toxoid IgG1
• Reactivity: Monkey
• Range: 0.078-2.500 1g/ml

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Please note – this product replaces our old product number IRKTAH3041 (same product, new number).